期刊
MACROMOLECULAR BIOSCIENCE
卷 13, 期 7, 页码 938-945出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201300039
关键词
bioconjugation; expressed protein ligation; polymersome decoration; strain-promoted azide alkyne cycloaddition; tumor-targeting polymersomes
资金
- Dutch Science Foundation (NWO)
- NRSCC
- Stichting Vanderes
Targeted carrier systems (e.g., liposomes or nanoparticles) are used to specifically deliver drugs to a site of interest. Site-direction can be achieved by attachment of targeting molecules, such as peptides, DNA/RNA, or antibodies, to the surface of the carrier. Here, the formation of polymersomes with tumor-targeting potential is described. A single-domain antibody (A12) that specifically targets PlexinD1 (a transmembrane protein overexpressed in tumor vasculature) is equipped with an azide-functionality using expressed protein ligation. This azide-containing A12 can subsequently be attached to BCN-functionalized polymersomes using a strain-promoted azide alkyne cycloaddition, thereby forming polymersomes with tumor-targeting potential.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据