期刊
MACROMOLECULAR BIOSCIENCE
卷 13, 期 8, 页码 1100-1109出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201200472
关键词
biopolymers; chitosan; mesenchymal stem cells (MSCs); microwell chips; spheroids
资金
- Program for Stem Cell and Regenerative Medicine Frontier Research [NSC101-2321-B-002-039]
- National Science Council, Taiwan, R.O.C.
Microwell chips (25mmx25mm) are fabricated to select proper substrates for growing three-dimensional (3D) spheroids from mesenchymal stem cells (MSCs). Different bio-macromolecules and their combinations are immobilized on the chip by air plasma treatment and by polyelectrolyte interaction. Only a small number of MSCs (approximate to 10(5)) are needed for each chip. The expression level of N-cadherin, a cell-adhesion molecule, is used as an indicator for cell-cell interactions. MSC spheroids expressing the highest N-cadherin level also show the greatest osteogenic potential. The microwell chip may be used as an efficient platform to screen bio-macromolecules that enhance the differentiation potential of MSCs.
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