期刊
MACROMOLECULAR BIOSCIENCE
卷 11, 期 5, 页码 662-672出版社
WILEY-BLACKWELL
DOI: 10.1002/mabi.201000402
关键词
cancer; down-regulation; polymers; self-assembly; siRNA
资金
- Alberta Advanced Education and Technology
- Canadian Institute of Health Research (CIHR)
- Natural Science and Engineering Research Council of Canada (NSERC)
- AHFMR
Characterization of a polymer library engineered to enhance their ability to protect and deliver their nucleotide cargo to the cells is reported. The zeta-potential continuously increased with higher polymer: siRNA weight ratio, and the zeta-potential of lipid-modified polymers: siRNA complexes were higher than PEI2 at all ratios. At polymer: siRNA ratio of 1: 1, all lipid-substituted polymers showed complete protection against degradation. Lipid-modified polymers significantly increased the cellular uptake of siRNA complexes and down-regulation of GAPDH and P-gp (max. 66% and 67%, respectively). The results indicate that hydrophobic modification of low molecular PEI could render this otherwise ineffective polymer to a safe effective delivery system for intracellular siRNA delivery and protein silencing.
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