期刊
MACROMOLECULAR BIOSCIENCE
卷 11, 期 10, 页码 1387-1392出版社
WILEY-BLACKWELL
DOI: 10.1002/mabi.201100113
关键词
cellular uptake; cellulose nanoparticles; fluorescently labeled; laser scanning microscopy; trimethylsilylcellulose
资金
- European Community [214653]
- Federal Ministry of Food, Agriculture and Consumer Protection
- German Agency of Renewable Resources [22021905]
Pure, perfectly spherical cellulose nanoparticles with sizes of approximate to 80-260nm can be prepared by dialysis starting from trimethylsilylcellulose (TMSC). The aqueous suspensions obtained are storable for several months. Subsequent covalent labeling of the cellulose nanoparticles with FITC has no influence on particle size, shape, and stability. The particles can be sterilized and suspended in biological media without structural changes. Incorporation of FITC-labeled cellulose nanoparticles into living human fibroblasts is studied using confocal LSM. In contrast to cellulose nanocrystals, fast cellular uptake is found for the nanospheres without transfection reagents or attachment of a receptor molecule. This suggests an influence of the geometry of biocompatible nanomaterials on endocytosis.
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