期刊
MACROMOLECULAR BIOSCIENCE
卷 10, 期 4, 页码 404-414出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200900300
关键词
bioreducible; dendrimers; gene delivery; graft copolymers; poly(amidoamine)
资金
- National Natural Science Foundation of China [20674058, 20874076]
- National Basic Research Program of China [2005CB623903, 2009CB930300]
- Ministry of Education of China
Dendritic poly(amidoamine)s (PAMAM)s were introduced into the side chains of disulfide-containing poly(amidoamine)s via repetitive Michael addition and amidation. The bioreducible poly(amidoamine)s grafted with dendritic polyamidoamines showed high buffer capacity, low cytotoxicity and strong DNA binding ability at low N/P ratio. They were able to condense DNA into small sized polycation/DNA complexes, which degraded and released the incorporated DNA under reductive conditions. In comparison to the original disulfide-containing poly(amidoamine) with aminoethyl side chain, the grafting of the bioreducible poly(amidoamine) with dendrimer greatly improved the transfection efficiencies of 293T and He La cells with foreign DNA at various N/P ratios. The structure gene delivery property relations of dendrimer-grafted polycations will provide valuable insight into the design of highly efficient and less toxic polycationic gene carriers.
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