期刊
MACROMOLECULAR BIOSCIENCE
卷 10, 期 12, 页码 1456-1464出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201000176
关键词
conducting polymers; peptides; polypyrroles; thin films; tissue engineering
资金
- National Science Foundation [0748001]
- Department of Defense TATRC [WB1XWH-07-1-0716]
- National Institute of Health [R01NS062019]
- NIH [EB006161]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [0748001] Funding Source: National Science Foundation
PPy is a conducting polymer material that has been widely investigated for biomedical applications. hESCs and adult rNSCs were grown on four PPy surfaces doped with PSS or peptide from laminin (p20, p31, and a mixture of p20 and p31) respectively. After 7 d, both PPy/p20 and PPy/p31 promoted neuroectoderm formation from hESCs. After 14 d of culture, surfaces containing p20 showed the highest percentage of neuronal differentiation from hESC, while the PPy/p31 surface showed better cell attachment and spreading. In rNSCs cultures, a higher percentage of neurons were found on the PPy/p20 surface than other surfaces at 7 and 14 d. For differentiated neurons, p20 promoted both the primary and total neurite outgrowth. Longer primary neurites were found on p20-containing surfaces and a longer total neurite length was found on PPy/p20 surface. These results demonstrated that, by doping PPy with different bioactive peptides, differentiation of stem cells seeded at different stages of development is affected.
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