期刊
MACROMOLECULAR BIOSCIENCE
卷 10, 期 12, 页码 1444-1455出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201000193
关键词
biomaterials; crosslinking; layer-by-layer; protein adsorption; QCM-D
资金
- Portuguese Foundation for Science and Technology (FCT) [PTDC/FIS/68209/2006]
- POCTI
- FEDER
- European FP7 Project Find and Bind [NMP4-SL-2009-229292]
- Fundação para a Ciência e a Tecnologia [PTDC/FIS/68209/2006] Funding Source: FCT
The adsorption of HSA onto CHI/ALG multilayer assemblies was assessed in situ using QCM-D. It was found that the behavior of HSA on biomaterials surface can be tuned by adjusting parameters of the polyelectrolyte system such as pH, layer number, crosslinker and polymer terminal layer. Our results confirmed the key role of electrostatic interactions during HSA adsorption, since oppositely charged surfaces were more effective in promoting protein adhesion. QCM-D data revealed that crosslinking (CHI/ALG)(5)CHI films allows HSA to become adsorbed in physiological conditions. Our results suggested that the biological potential of biopolymers and the mild conditions of the LbL technique turn these natural nanoassemblies into a suitable choice to be used as pH-sensitive coatings.
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