期刊
MACROMOLECULAR BIOSCIENCE
卷 10, 期 7, 页码 768-774出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200900396
关键词
differential scanning calorimetry (DSC); glycogen phosphorylase b; GroEL; thermoaggregation; ultracentrifugation
资金
- Russian Foundation for Basic Research [08-04-00666-a, 08-04-00231, 08-08-00540]
- Russian Academy of Sciences
The suppression of the thermal aggregation of glycogen phosphorylase b (Phb) from rabbit skeletal muscle by the chaperonin GroEL is studied using dynamic light scattering. It is shown that the decrease in the rate of Phb aggregation under the action of GroEL is due to the transition of the aggregation process from the kinetic regime, wherein the rate of aggregation is limited by diffusion of the interacting particles, to a 20000 regime where the sticking probability for the colliding particles becomes lower than one (reaction-limited cluster-cluster aggregation). The analytical-ultracentrifugation data show that elevated temperatures induce dissociation of the dimeric Phb. The formation of a complex between the denatured monomeric form of Phb and the dissociated forms of GroEL is detected during heating at 46 degrees C.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据