期刊
MACROMOLECULAR BIOSCIENCE
卷 10, 期 2, 页码 202-210出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200900230
关键词
gene delivery; graft copolymers; polyelectrolytes; stimuli-sensitive polymers; temperature-responsive polymer
资金
- National Natural Science Foundations of China [90403140]
A temperature-responsive polymer poly{2-(dimethylamino) ethyl methacrylate-co-[cis-butenedioic anhydride -poly[(N-isopropylacrylamide)-co-(butyl methacrylate)]]} (PDMNIB) was synthesized by free radical polymerization. The polymer had a significant temperature-responsive behavior with a lower critical solution temperature (LCST) at 20 degrees C. Gel retardation assay showed that PDMNIB could efficiently interact with DNA. Dynamic light scattering (DLS) and zeta potential measurement indicated that the average sizes and the surface electric charges of the PDMNIB/DNA complexes could be changed by temperature. Due to the thermosensitive interaction between PDMNIB and DNA, the gene transfection efficiency of PDMNIB could be improved by temperature.
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