期刊
MACROMOLECULAR BIOSCIENCE
卷 9, 期 6, 页码 568-574出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200800334
关键词
disulfide crosslinkage; electrospinning; fibers; gamma-PGA; tissue engineering
资金
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [B/18700426]
- Japanese Society for Artocial Organs
- New Energy and Industrial Technology Development Organization (NEDO) of Japan [061344017a]
- COE program Center for Integrated Cells and Tissue Regulation
- Deutsche Forschungsgemeinschaft [SFB/TR 37]
Novel water-insoluble, and reduction-responsive nonwoven scaffolds were fabricated from gamma-PGA and tested in cell culture. An electrospinning method was developed to produce scaffolds of fibers with diameters of 0.05-0.5 mu m. Crosslinking of the fibers with cystamine in the presence of EDC resulted in water-insoluble gamma-PGA nonwovens with disulfide crosslinkages. These crosslinked fibers were easily decomposed under physiological conditions using L-cysteine, a biocompatible reductant. In vitro experiments with mouse L929 fibroblasts showed good adhesion onto gamma-PGA-SS fiber matrices and excellent cell proliferation. These gamma-PGA-SS nonwovens can be used as novel biocompatible and biodegradable scaffolds with reduction-responsiveness for biomedical or tissue engineering applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据