期刊
MACROMOLECULAR BIOSCIENCE
卷 9, 期 5, 页码 421-428出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200800273
关键词
hemoglobin; hydrogels; molecularly imprinting; myoglobin; preparation temperature
Temperature-sensitive imprinted and non-imprinted hydrogels composed of N-isopropylacrylamide (NIPA) and 2-acrylamido-2-methyl-propanosulfonic acid (AMPS) have been prepared by free-radical crosslinking copolymerization in aqueous solution at three different temperatures: 10 T (below the lower critical solution temperature, LCST), 33 degrees C (at the LCST), and 40 degrees C (above the LCST). Myoglobin (Mb, MW 17 kDa) is used as the template biomolecule. The effects of the initial concentration and adsorption time over the Mb adsorption capacity of the hydrogels have been analyzed and found to be strongly dependent on the preparation temperature (T-prep). The maximum Mb adsorption for the imprinted hydrogel prepared at 10 degrees C is 97.40 +/- 2.35 mg Mb . g(-1) dry gel in 0.32 mg.mL(-3), Mb solution at 22 degrees C. Moreover, batch adsorption equilibrium and selectivity studies have been performed using a reference molecule, hemoglobin (Hb, MW 65 kDa). The imprinted hydrogels have a 2.8-3.3 times higher adsorption capacity for Mb than the non-imprinted hydrogels prepared at the same T(prep)s, and also have a 1.8-2.7 times higher selectivity for the imprinted molecule.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据