期刊
MABS
卷 5, 期 3, 页码 491-500出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/mabs.24245
关键词
aggregation; plasma; compatibility; biopharmaceuticals; administration
Analytical methods based on light microscopy, 90 degrees light-scattering and surface plasmon resonance (SPR) allowed the characterization of aggregation that can occur when antibodies are mixed with human plasma. Light microscopy showed that aggregates formed when human plasma was mixed with 5% dextrose solutions of Herceptin((R)) (trastuzumab) or Avastin((R)) (bevacizumab) but not Remicade((R)) (infliximab). The aggregates in the plasma-Herceptin((R))-5% dextrose solution were globular, size range 0.5-9 m, with a mean diameter of 4 m. The aggregates in the plasma-Avastin((R))-5% dextrose samples had a mean size of 2 m. No aggregation was observed when 0.9% NaCl solutions of Herceptin((R)), Avastin((R)) and Remicade((R)) were mixed with human plasma. 90 degrees light-scattering measurements showed that aggregates were still present 2.5 h after mixing Herceptin((R)) or Avastin((R)) with 5% dextrose-plasma solution. A SPR method was utilized to qualitatively describe the extent of interactions of surface-bound antibodies with undiluted human serum. Increased binding was observed in the case of Erbitux((R)) (cetuximab), whereas no binding was measured for Humira((R)) (adalimumab). The binding of sera components to 13 monoclonal antibodies was measured and correlated with known serum binding properties of the antibodies. The data presented in this paper provide analytical methods to study the intrinsic and buffer-dependent aggregation tendencies of therapeutic proteins when mixed with human plasma and serum.
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