Comparison of rheological properties of wet gluten: Creep-recovery and biaxial compression

The objective of this study is to examine the fundamental theological properties of gluten extracted from six commercial wheat flours and four gluten products (GA, GB, GC and GD) at two substitution levels (3 and 6 g/100 g). Experimental data from creep-recovery (shear deformation, 100 Pa) and compression-recovery (biaxial compression, 8 N) tests were fitted into a Burgers model. GA and GC were associated with variation in elastic recovery (strength) and zero-shear viscosity (related to molecular weight) while CB and GD with variation in softening effect that would result in improved extensibility. Differences in composition based on mass-to-charge ratio suggested that GA and GC can form longer polymer chains and have higher LMW-GS and LMW-GS/HMW-GS ratio compared to GB and GD, which form shorter polymer chains. Redundancy analysis clearly separated the effect of gluten substitutions and levels across the six flours in terms of strength and deformability of the structure.