期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 43, 期 6, 页码 964-970出版社
ELSEVIER
DOI: 10.1016/j.lwt.2010.02.005
关键词
Biofilm; Lettuce; Escherichia coli; Listeria monocytogenes; SEM
资金
- TUBITAK
- European Community [FP6-FOOD-CT-2003-506358]
The effects of ozone (2 mg/L), chlorine (100 mg/L) and organic acid (0.25 g/100 g citric acid plus 0.50 g/100 g ascorbic acid) treatments at 10 degrees C for 2 min on the removal of Escherichia coli and Listeria monocytogenes cells embedded inside biofilms on the surface of lettuce leaves were studied. None of the sanitizing treatments were found effective in removing the bacterial biofilms. Initiation of biofilms was observed after 24 h of incubation. Bacterial cells appeared as individual cells, rather than clusters after 6 h incubation, thus 99.9% reductions in both E. coli and L monocytogenes counts were achieved with all the three treatments. However, after 48 h incubation, none of the treatments resulted in higher than 90% reduction in microbial counts. Biofilm formation was demonstrated for the 48 h incubated samples with SEM images. (C) 2010 Elsevier Ltd. All rights reserved.
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