期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 41, 期 6, 页码 1082-1088出版社
ELSEVIER
DOI: 10.1016/j.lwt.2007.07.005
关键词
Rhodobacter sphaeroides; carotenoids; optimization; extraction; Box-Behnken design
The comparison of the three methods ultrasonic assisting, grinding and HCl assisting on carotenoids extraction yield from Rhodobacter sphaeroides (R. sphaeroides) was carried out. The HCl-assisted extraction was found to be the most effective. And then, based on HCl-assisted extraction method, the effect of temperature, solvent-solid ratio and duration on the yield of carotenoids extracted from R sphaeroides was investigated using single factor and Box-Behnken experimental design. The optimal conditions for HCl-assisted extraction of carotenoids from R sphaeroides were found to be: temperature 30 degrees C, solvent-solid ratio 40 ml/g, duration 40 min. The first two extractions were enough to approximately exhaust the entire content of carotenoids in R. sphaeroides. (c) 2007 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.
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