期刊
LUMINESCENCE
卷 29, 期 6, 页码 579-585出版社
WILEY-BLACKWELL
DOI: 10.1002/bio.2584
关键词
capillary electrophoresis; chemiluminescence; hemin; phenol detection
资金
- national program on development of scientific instruments and equipment [2011YQ150072]
- Natural Science Fund of Anhui Education Department [KJ2012B059]
Based on the catalytic activity of hemin, an efficient biocatalyst, an indirect capillary electrophoresis-chemiluminescence (CE-CL) detection method for phenols using a hemin-luminol-hydrogen peroxide system was developed. Through a series of static injection experiments, hemin was found to perform best in a neutral solution rather than an acidic or alkaline medium. Although halide ions such as Br- and F- could further enhance the CL signal catalyzed by hemin, it is difficult to apply these conditions to this CE-CL detection system because of the self-polymerization of hemin, as it hinders the CE process. The addition of concentrated ammonium hydroxide to an aqueous/dimethyl sulfoxide solution of hemin-luminol afforded a stable CE-CL baseline. The indirect CE-CL detection of five phenols using this method gave the following limits of detections: 4.8 x 10(-8) mol/L (o-sec-butylphenol), 4.9 x 10(-8) mol/L (o-cresol), 5.4 x 10(-8) mol/L (m-cresol), 5.3 x 10(-8) mol/L (2,4-dichlorophenol) and 7.1 x 10(-8) mol/L (phenol). Copyright (c) 2013 John Wiley & Sons, Ltd.
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