期刊
LUMINESCENCE
卷 24, 期 2, 页码 102-107出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/bio.1080
关键词
chemiluminescence; thiosemicarbazide; ampicillin sodium; amoxicillin; non-aqueous solvent effect; surfactant effect
An enhanced thiosemicarbazide(TSC)-H(2)O(2), chemiluminescence (CL) system was established and proposed as a new analytical method for determination of beta-lactam antibiotics, ampicillin sodium and amoxicillin at microgram levels. The method is based on the inhibition of CL emission accompanying oxidation of TSC by H(2)O(2) in alkaline medium. The effect of anionic, cationic, and non-ionic surfactants on the CL emission of the system was studied. Both N-cetyl-N,N,N-trimethylammonium bromide (CTMAB) and Triton X-100, unlike sodium dodecyl sulfate (SDS), reinforced the CL intensity and were efficient to approximately the same level. The effect of the presence of eight non-aqueous solvents on the CL system was also investigated. Upon addition of both of the non-ionic surfactant, Triton X-100, and the non-aqueous solvent, N,N-dimethyl formamide (DMF), the intensity of the CL reaction was increased 100-fold. This method allows the measurement of 25-545 mu g amoxicillin, and 35-350 mu g ampicillin sodium. The detection limits are 8 mu g for amoxicillin and 9 mu g for ampicillin sodium. The relative standard deviations of six replicate measurements of 200 mu g amoxicillin and 200 mu g ampicillin sodium were 1.9 and 2.1 %, respectively. The effect of foreign species on the determination of amoxicillin and ampicillin sodium was also examined. The proposed method was successfully applied to the determination of ampicillin sodium and amoxicillin in some pharmaceutical dosage forms. Copyright (C) 2008 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据