期刊
RADIOTHERAPY AND ONCOLOGY
卷 116, 期 3, 页码 438-442出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.radonc.2015.06.019
关键词
Cancer; ED-B; Immunotherapy; F9; MHCI
资金
- Philogen S.p.A. (Sovicille, Italy), 7th framework program (METOXIA)
- Netherlands Organisation for Scientific Research (NWO) [911-06-003]
Background and purpose: Recently, we have shown that radiotherapy (RT) combined with the immunocytokine L19-1L2 can induce long-lasting antitumour effects, dependent on ED-B expression and infiltration of cytotoxic T cells. On the other hand, in certain tumours, IL2 treatment can trigger a natural killer cell (NK) immune response. The aim of this study is to investigate the therapeutic effect of our combination therapy in the ED-B positive F9 teratocarcinoma model, lacking MHCI expression and known to be dependent on NK immune responses. Material and methods: In syngeneic F9 tumour bearing 129/FvHsd mice tumour growth delay was evaluated after local tumour irradiation (10 Gy) combined with systemic administration of L19-1L2. Immunological responses were investigated using flow cytometry. Results: Tumour growth delay of L19-1L2 can be further improved by a single dose of RT administered before immunotherapy, but not during immunotherapy. Furthermore, treatment of L19-1L2 favours a NK response and lacks cytotoxic T cell tumour infiltrating immune cells, which may be explained by the absence of MHCI expression. Conclusion: An additive effect can be detected when the NK dependent F9 tumour model is treated with radiotherapy and L19-11.2 and therefore this combination could be useful in the absence of tumoural MHCI expression. (C) 2015 The Authors. Published by Elsevier Ireland Ltd. Radiotherapy and Oncology 116 (2015) 438-442 This is an open access article under the CC BY-NC-ND license (http://creativecommons.orgflicenses/by-nc-nd/4.0/).
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