期刊
LIPIDS
卷 47, 期 2, 页码 185-193出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11745-011-3602-9
关键词
Steryl glucosides; Acyl steryl glucosides; Sterols; Electrospray ionization tandem mass spectrometry; Gas chromatography; Arabidopsis thaliana; UDP-glucose:sterol glucosyltransferase; ugt80A2,B1, UGT80A2, UGT80B1
资金
- National Research Initiative from the United States Department of Agriculture National Institute of Food and Agriculture [2007-35304-18453]
- National Science Foundation [MCB 0517758, EPS 0236913, MCB 0455318, 0920663, DBI 0521587]
- Kansas Technology Enterprise Corporation
- Kansas IDeA Network of Biomedical Research Excellence (K-INBRE) of National Institutes of Health [P20RR16475]
- Kansas State University
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1122016, 0920663] Funding Source: National Science Foundation
Establishment of sensitive methods for the detection of cellular sterols and their derivatives is a critical step in developing comprehensive lipidomics technology. We demonstrate that electrospray ionization tandem (triple quadrupole) mass spectrometry (ESI-MS/MS) is an efficient method for monitoring steryl glucosides (SG) and acyl steryl glucosides (ASG). Comparison of analysis of SG and ASG by ESI-MS/MS with analysis by gas chromatography with flame ionization detection (GC-FID) shows that the two methods yield similar molar compositions. These data demonstrate that ESI-MS/MS response per molar amount of sterol conjugate is similar among various molecular species of SG and ASG. Application of ESI-MS/MS to seed samples from wild-type Arabidopsis and a mutant deficient in two UDP-glucose:sterol glucosyltransferases, UGT80A2 and UGT80B1, revealed new details on the composition of sitosteryl, campesteryl and stigmasteryl glucosides and ASG. SG were decreased by 86% in the ugt80A2,B1 double mutant, compared to the wild-type, while ASG were reduced 96%. The results indicate that these glucosyltransferases account for much of the accumulation of the sterol conjugates in wild-type Arabidopsis seeds.
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