期刊
LIPIDS
卷 44, 期 9, 页码 777-785出版社
WILEY
DOI: 10.1007/s11745-009-3322-6
关键词
Marine two-headed sphingolipid-like compound; Anticarcinogenic properties; Tumor cells; p53-dependent apoptosis
资金
- Russian Science Schools, Program of Presidium of RAS [2813.2008.4]
- RFFI [RFFI 09-04-00015-a]
- FEB RAS [09-III-A-05-139, 09-III-A-05-146]
- KOSEF
Rhizochalin [(2R,3R,26R,27R)-2,27-diamino-3-hydroxy-26-[(2R,3R,4S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yloxy]octacosan-11-one], an antimicrobial and cytotoxic marine two-headed sphingolipid-like natural product, isolated from the sponge Rhizochalina incrustata, and some related compounds were studied as anticarcinogenic and proapoptotic agents. The corresponding effects were tested on the mouse skin epidermal JB6 P+ Cl 41 cell line, its stable transfectants, THP-1, HeLa, and SNU-C4 human tumor cells using a variety of assessments, including cell viability (MTS), flow cytometry, anchorage-independent soft agar, and luciferase assays. At 5-10 mu M concentrations, rhizochalin was effective as an inhibitor of the malignant transformation of JB6 P+ Cl 41 cells or colony formation of human tumor cells, which exerted its action, at least in part, through the induction of p53-dependent apoptosis. Structure-activity relationship study showed aglycon of rhizochalin to be the most active while peracetylated aglycon was the least active among the compounds studied.
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