期刊
LIFE SCIENCES
卷 93, 期 9-11, 页码 388-392出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2013.07.009
关键词
Food flavoring agent; Brain development; Brain electrophysiology; Glutamatergic system; Rat
资金
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES-Procad) [131/2007]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (Facepe-IBPG) [0085-2.07/09]
- Instituto Brasileiro de Neurociencias (IBN-Net/Finep) [4191]
- Instituto Nacional de Neurociencia Translacional (INCT) [573604/2008-8]
Aims: Monosodium glutamate (MSG) is a neuroexcitatory amino add used in human food to enhance flavor. MSG can affect the morphological and electrophysiological organization of the brain. This effect is more severe during brain development. Here, we investigated the electrophysiological and morphological effects of MSG in the developing rat brain by characterizing changes in the excitability-related phenomenon of cortical spreading depression (CSD) and microglial reaction. Main methods: From postnatal days 1-14, Wistar rat pups received 2 or 4 g/kg MSG (groups MSG-2 and MSG-4, respectively; n = 9 in each group), saline (n = 10) or no treatment (naive group; n = 5) every other day. At 45-60 days, CSD was recorded on two cortical points for 4 h. The CSD parameters velocity, and amplitude and duration of the negative potential change were calculated. Fixative-perfused brain sections were immunolabeled with anti-IBA-1 antibodies to identify and quantify cortical microglia. Key findings: MSG-4 rats presented significantly higher velocities (4.59 +/- 0.34 mm/min) than the controls (saline, 3.84 +/- 020 mm/min; naive, 3.71 +/- 0.8 mm/min) and MSG-2 group (3.75 +/- 0.10 mm/min). The amplitude (8.8 +/- 22 to 11.2 +/- 1.9 mV) and duration (58.2 +/- 7.1 to 73.6 +/- 6.0 s) of the negative slow potential shift was similar in all groups. MSG-treatment dose-dependently increased the microglial immunolabeling. Significance: The results demonstrate a novel, dose-dependent action of MSG in the developing brain, characterized by acceleration of CSD and significant microglial reaction in the cerebral cortex. The CSD effect indicates that MSG can influence cortical excitability, during brain development, as evaluated by CSD acceleration. Data suggest caution when consuming MSG, especially in developing organisms. (C) 2013 Elsevier Inc. All rights reserved.
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