期刊
LIFE SCIENCES
卷 91, 期 17-18, 页码 866-871出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2012.08.035
关键词
Alpha-pinene; Acute pancreatitis; Cerulein; Cytokines
资金
- Regional Innovation System of the Ministry of Knowledge Economy [B55-122285]
- National Research Foundation of Korea (NRF)
- Korean government (MEST) [2010-0029498]
Aims: Acute pancreatitis (AP) is a complicated inflammatory disease that has an unknown underlying pathogenesis. Because alpha-pinene can modulate inflammation, we examined whether alpha-pinene plays a role in AP. Main methods: Alpha-pinene was administered intraperitoneally 1 h prior to the first injection of cerulein. Once AP developed, cerulein, a stable cholecystokinin analog, was injected hourly over a 6-h period. Blood samples were taken 6 h later to determine serum amylase and lipase levels. The pancreas and lungs were rapidly removed for morphological examination, myeloperoxidase assay, and real-time reverse transcription polymerase chain reaction. We also isolated the pancreatic acinar cells using a collagenase solution. Cell viability, and cytokine productions were measured in pancreatic acini. Key findings: Intraperitoneal administration of alpha-pinene reduced the pancreatic weight (PW) to body weight (BW) ratio and the serum levels of amylase and lipase. Alpha-pinene treatment also reduced histological damage and myeloperoxidase activity in the pancreas and lungs. Furthermore, alpha-pinene pretreatment reduced the production of pancreatic tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and IL-6 during cerulein-induced AP. In vitro, alpha-pinene inhibited cerulein-induced cell death and cytokine production in isolated cerulein-treated pancreatic acinar cells. Significance: These findings suggest that alpha-pinene has an anti-inflammatory effect during cerulein-induced AP. (C) 2012 Elsevier Inc. All rights reserved.
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