期刊
LIFE SCIENCES
卷 89, 期 15-16, 页码 577-583出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2011.05.017
关键词
Pre-pubertal rat testes; Sertoli cells; Membrane potential; Follicle stimulating hormone; Testosterone; K-ATP(+) channels; L-type calcium channels
资金
- CNPq
- FAPERGS [ARD 0903278]
This minireview describes the rapid signaling actions of follicle stimulating hormone (FSH) and testosterone in immature Sertoli cells mainly related to Ca2+ inflow and the electrophysiological changes produced by hormones. The rapid membrane actions of FSH occur in a time frame of seconds to minutes, which include membrane depolarization and the stimulation of Ca-45(2+) uptake. These effects can be prevented by pertussis toxin (PTX), suggesting that they are likely mediated by Gi-protein coupled receptor activation. Furthermore, these effects were inhibited by verapamil, a blocker of the L-type voltage-dependent Ca2+ channel (VDCC). Finally, FSH stimulation of Ca-45(2+) uptake was inhibited by the (phosphoinositide 3-kinase) PI3K inhibitor wortmannin. These results suggest that the rapid action of FSH on L-type Ca2+ channel activity in Sertoli cells from pre-pubertal rats is mediated by the Gi/G beta gamma/PI3K gamma pathway, independent of its effects on insulin-like growth factor type I (IGF-I). Testosterone depolarizes the membrane potential and increases the resistance and the Ca-45(2+) uptake in Sertoli cells of the seminiferous tubules of immature rats. These actions were nullified by diazoxide (K-ATP(+) channel opener). Testosterone actions were blocked by both PTX and the phospholipase C (PLC) inhibitor U73122, suggesting the involvement of PLC - phosphatidylinositol 4-5 bisphosphate (PIP2) hydrolysis via the Gq protein in the testosterone-mediated pathway. These results indicate that testosterone acts on the Sertoli cell membrane through the K-ATP(+) channels and PLC-PIP2 hydrolysis, which closes the channel, depolarizes the membrane and stimulates Ca-45(2+) uptake. These results demonstrate the existence of rapid non-classical pathways in immature Sertoli cells regulated by FSH and testosterone. (C) 2011 Elsevier Inc. All rights reserved.
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