期刊
LIFE SCIENCES
卷 84, 期 13-14, 页码 394-401出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2008.11.017
关键词
Smooth muscle 22 alpha; F-actin; Cytoskeleton; Phenotype; Vascular smooth muscle cells
资金
- National Natural Science Foundation of China [30570661, 30670845]
- Major State Basic Research Development Program of China [2008CB517402]
- Natural Science Foundation of Hebei Province, China [C2007000831]
Aim: Smooth muscle 22 alpha (SM22 alpha) is not required for the development and basal homeostatic function of smooth muscle cells (SMCs). However, a recent study demonstrated that SM22 alpha plays a role in inhibiting the phenotypic modulation of vascular SMCs (VSMCs) from contractile to synthetic/proliferative cells. The present study investigated the mechanism underlying the SM22 alpha-mediated maintenance of the contractile phenotype of VSMCs. Main methods: The redifferentiation of synthetic SMCs was induced by serum deprivation for 48-72 h. The expression plasmids containing full-length cDNA of rat SM22 alpha and a vector expressing SM22 alpha antisense transcripts were constructed, respectively. Coimmunoprecipitation, cosedimentation assay and immunofluorescence analyses were used to detect the interaction of SM22 alpha with F-actin. Key findings: The results revealed that SM22 alpha directly interacted and colocalized with F-actin and thus participated in the organization of the actin cytoskeleton in differentiated VSMCs. SM22 alpha facilitated the assembly of actin filaments into bundles. The blockade of SM22 alpha expression by SM22 alpha antisense RNA led to the thinning and dispersion of actin filaments. Consequently, the ratio of F-actin to globular (G)-actin was reduced, and the cell contractility was lost. Significance: The SM22 alpha-induced F-actin bundling enhances the contractility and mobility of VSMCs, and the activity of SM22 alpha is necessary for maintaining the differentiated phenotype of VSMCs. (C) 2008 Published by Elsevier Inc.
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