期刊
LIFE SCIENCES
卷 85, 期 23-26, 页码 782-787出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2009.10.009
关键词
Nitric oxide; Serotonin; Gastric vagal afferent; Enterochromaffin cells
Aim: We previously reported the possible involvement of mucosal nitric oxide (NO)-triggered 5-HT release in luminal glutamate sensing by the gastric vagus nerve, and we proposed that the stomach, like the duodenum, could taste luminal nutrients. Nitric oxide synthase (NOS) is widely distributed in the gastric mucosa, but the physiological role of mucosal NO is not well understood. In this study, we investigated the functional coupling of NO and vagal nerve endings in the gastric mucosa. Main methods: For electrophysiological recordings, male Sprague-Dawley rats were anesthetized with urethane, and afferent nerve responses of rat vagal gastric branches to a NO donor were monitored. Key findings: Intravenous application of 100 mu g/kg sodium nitroprusside (SNP) transiently increased afferent nerve discharges of the rat ventral gastric vagus, which was followed by rapid changes in blood pressure. High doses of SNP (>300 mu g/kg. i.v.) showed a biphasic increase in afferent discharges. Secondary activation of the vagal afferent continued even after blood pressure returned to basal levels. SNP-evoked afferent responses were abolished by mucosal 5-HT depletion using p-cholorophenylalanine and were inhibited by pre- and post-treatment with the 5-HT3 antagonist granisetron. Significance: These pharmacological results strongly indicate that NO-triggered 5-HT release is coupled to vagal afferent activation in the rat gastric mucosa. Crown Copyright (C) 2009 Published by Elsevier Inc. All rights reserved.
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