期刊
LEUKEMIA RESEARCH
卷 37, 期 5, 页码 580-585出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.leukres.2013.01.015
关键词
Leukemia; WT1; RNA therapy; tRNase Z(L); Small guide RNA
资金
- Adaptable and Seamless Technology Transfer Program through Target-driven R&D, Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research [24591385] Funding Source: KAKEN
TRUE gene silencing is a technology to eliminate specific cellular RNAs by using tRNase Z(L) and small guide RNA (sgRNA). Here we investigated how WT1-mRNA-targeting sgRNAs affect leukemic cells. We showed that sgRNA can be easily taken up by cells without any transfection reagents, and that the naked sgRNAs targeting the WT1 mRNA can reduce its mRNA levels and WT1 protein amounts in the WT1-expressing leukemic cells. Concomitantly, these sgRNAs efficiently induced apoptosis in these cells but not in WT1-nonexpressing cells. We also demonstrated that the reduction in the WT1 mRNA level is caused by its cleavage by tRNase Z(L). (C) 2013 Elsevier Ltd. All rights reserved.
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