期刊
LEUKEMIA RESEARCH
卷 33, 期 9, 页码 1224-1232出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.leukres.2009.03.047
关键词
Plasmacytoid dendritic cell leukemia; Toll-like receptors; IFN-alpha; IL-12; IRF1; IRF7; Antigen presenting ability; Antigen uptake
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [11670986, 12217048]
- Grants-in-Aid for Scientific Research [12217048, 11670986] Funding Source: KAKEN
We established a plasmacytoid dendritic cell (pDC) line (PMDC05) from leukemia cells of pDC leukemia. PMDC05 cells were positive for CD4, CD56, CD33, HLA-DR, CD123 (IL-3R alpha) and CD86 in the absence of lineage markers. mRNA of TLR1, TLR2, TLR4, TLR7 and TLR9 was clearly expressed and among these TLRs, TLR7 was prominent. Production of IFN-alpha and IL-12 in PMDC05 was enhanced by the stimulation with CpG-A and LPS, respectively. PMDC05 possessed a considerable antigen presenting ability, which was enhanced by culturing with IL3, influenza virus or LPS. PMDC05 could be a useful tool for investigating the pathophysiology of pDCL. (C) 2009 Elsevier Ltd. All rights reserved.
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