期刊
LEUKEMIA
卷 27, 期 1, 页码 142-149出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/leu.2012.216
关键词
chronic lymphocytic leukaemia; minimal residual disease; flow cytometry
资金
- IntraSure
- Cariplo Foundation (Milan, Italy)
- Program Molecular Clinical Oncology-5 per mille number [9965]
- Associazione Italiana per la Ricerca sul Cancro (AIRC Milano, Italy)
- Progetti di Ricerca di Interesse Nazionale (PRIN - Ministry of education, University and Research - MIUR, Rome, Italy)
- Ricerca Finalizzata (Ministry of Health, Rome, Italy)
- LLR
Detection of minimal residual disease (MRD) in chronic lymphocytic leukaemia (CLL) is becoming increasingly important as treatments improve. An internationally harmonised four-colour (CLR) flow cytometry MRD assay is widely used but has limitations. The aim of this study was to improve MRD analysis by identifying situations where a less time-consuming CD19/CD5/kappa/lambda analysis would be sufficient for detecting residual CLL, and develop a six-CLR antibody panel that is more efficient for cases requiring full MRD analysis. In 784 samples from CLL patients after treatment, it was possible to determine CD19/CD5/kappa/lambda thresholds that identified cases with detectable MRD with 100% positive predictive value (PPV). However, CD19/CD5/kappa/lambda analysis was unsuitable for predicting iwCLL/NCI response status or identifying cases with no detectable MRD. For the latter cases requiring a full MRD assessment, a six-CLR assay was designed comprising CD19/CD5/CD20 with (1) CD3/CD38/CD79b and (2) CD81/CD22/CD43. There was good correlation between four-CLR and six-CLR panels in dilution studies and clinical samples, with 100% concordance for detection of residual disease at the 0.01% (10(-4)) level (n = 59) and good linearity even at the 0.001-0.01% (10(-5)-10(-4)) level. A six-CLR panel therefore provides equivalent results to the four-CLR panel but it requires fewer reagents, fewer cells and a much simpler analysis approach. Leukemia (2013) 27, 142-149; doi:10.1038/leu.2012.216
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