期刊
LEUKEMIA
卷 26, 期 4, 页码 757-768出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/leu.2011.256
关键词
acute myeloid leukemia; acute lymphoblastic leukemia; multiple myeloma; drug resistance; bortezomib; proteasome inhibition
资金
- Stichting Translational Research, Cancer Center Amsterdam
- NWO-AGIKO
- Royal Netherlands Academy of Arts and Sciences (KNAW)
- Netherlands Organization for Scientific Research (NWO)
- Dutch Foundation Children Cancer-free (KIKA)
Proteasome inhibition is a novel treatment for several hematological malignancies. However, resistance to the proteasome inhibitor bortezomib (BTZ, Velcade) is an emerging clinical impediment. Mutations in the beta 5 subunit of the proteasome, the primary target of BTZ, have been associated with drug resistance. However, the exact mechanism by which these mutations contribute to BTZ resistance, is still largely unknown. Toward this end, we here developed BTZ-resistant multiple myeloma (8226) and acute lymphoblastic leukemia (CCRF-CEM) cell line models by exposure to stepwise increasing concentrations of BTZ. Characterization of the various BTZ-resistant cells revealed upregulation of mutant beta 5 subunit of the proteasome. These newly identified beta 5-subunit mutations, along with previously described mutations, formed a mutation cluster region in the BTZ-binding pocket of the beta 5 subunit, that of the S1 specificity pocket in particular. Moreover, we provide the first evidence that the mechanism underlying BTZ resistance in these tumor cells is impaired binding of BTZ to the mutant beta 5 subunit of the proteasome. We propose that proteasome subunit overexpression is an essential compensatory mechanism for the impaired catalytic activity of these mutant proteasomes. Our findings further suggest that second-generation proteasome inhibitors that target the alpha 7 subunit of the proteasome can overcome this drug resistance modality. Leukemia (2012) 26, 757-768; doi:10.1038/leu.2011.256; published online 23 September 2011
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