期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 55, 期 3, 页码 182-188出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1472-765X.2012.03276.x
关键词
cell viability; propidium monoazide; real-time; qPCR
资金
- National Research Foundation of Korea
- Korean government [2011-0029826]
- Agriculture Research Center programme of the Ministry of Food, Agriculture, Forestry and Fisheries
- National Research Foundation of Korea [2010-0029113] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Aims: The ability to distinguish between viable and/or infectious micro-organisms and inactivated cells is extremely important for correctly performing microbial risk assessments. In this study, we evaluated whether propidium monoazide (PMA)-qPCR could distinguish between viable and nonviable bacteria and viruses. Methods and Results: A PMA-qPCR combined assay was applied to viable and inactivated bacteria (Escherichia coli and Bacillus subtilis) and viruses (MS2 and murine norovirus [MNV]). PMA, a DNA-intercalating agent, in combination with PCR was better able to distinguish between viable and nonviable bacteria and viruses than conventional PCR. Conclusions: These results suggest that a combined PMA-qPCR assay can be used to measure the viability of bacterial cells and bacteriophage MS2, but not MNV. Significance and Impact of the Study: PMA-qPCR could potentially be used to measure the viability of some micro-organisms, including virus. However, a thorough evaluation should be performed prior to measuring the viability of micro-organisms by PMA-qPCR in a quantitative way.
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