期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 56, 期 2, 页码 142-148出版社
WILEY-BLACKWELL
DOI: 10.1111/lam.12027
关键词
dairy; modelling; nonthermal processes; optimization
资金
- Tecnologico de Monterrey (Research Chair Funds) [CAT-200]
- CONACyT-SEP fund [101700]
- CONACyT [290505]
Multiple-pass ultrahigh pressure homogenization (UHPH) was used for reducing microbial population of both indigenous spoilage microflora in whole raw milk and a baroresistant pathogen (Staphylococcus aureus) inoculated in whole sterile milk to define pasteurization-like processing conditions. Response surface methodology was followed and multiple response optimization of UHPH operating pressure (OP) (100, 175, 250 MPa) and number of passes (N) (15) was conducted through overlaid contour plot analysis. Increasing OP and N had a significant effect (P < 0.05) on microbial reduction of both spoilage microflora and Staph. aureus in milk. Optimized UHPH processes (five 202-MPa passes; four 232-MPa passes) defined a region where a 5-log10 reduction of total bacterial count of milk and a baroresistant pathogen are attainable, as a requisite parameter for establishing an alternative method of pasteurization. Multiple-pass UHPH optimized conditions might help in producing safe milk without the detrimental effects associated with thermal pasteurization.
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