期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 51, 期 3, 页码 358-361出版社
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1472-765X.2010.02885.x
关键词
genomics; molecular genetics; staphylococci
资金
- Medical Research Council [G0501247]
- MRC [G0501247] Funding Source: UKRI
- Medical Research Council [G0501247] Funding Source: researchfish
Aims: To provide comparative genome sequence data for two related model strains of Staphylococcus aureus (SH1000 and 8325-4) that are used extensively in laboratory research. Methods and Results: Comparative genome sequencing was used to identify genetic differences between Staph. aureus SH1000 and the fully genome-sequenced ancestral strain, Staph. aureus NCTC 8325. PCR amplification and DNA sequencing were employed to determine which of the genetic polymorphisms identified were also present in Staph. aureus 8325-4, a direct derivative of 8325 and the parent strain of SH1000. Aside from known genetic differences between these strains, Staph. aureus SH1000 harboured 15 single-nucleotide polymorphisms compared with 8325 (of which 12 were also found in 8325-4), and a 63-bp deletion upstream of the spa gene not present in either 8325 or 8325-4. Conclusions: Staphylococcus aureus SH1000 and 8325-4 contain a number of genetic polymorphisms relative to the progenitor strain of the lineage (8325) and to each other. Significance and Impact of the Study: The comparative genome sequences of SH1000 and 8325-4 presented here define the genotypes of two key strains in staphylococcal laboratory research and reveal genetic polymorphisms that may impact their phenotypic properties.
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