期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 50, 期 2, 页码 237-240出版社
WILEY
DOI: 10.1111/j.1472-765X.2009.02775.x
关键词
chi-squared; cut-off; genome copy number; infectivity; statistical analysis
Aims: Classic virological tests are time consuming and labour-intensive; real-time RT-PCR has proven to be a fast method to detect and quantify enterovirus genomes in clinical and environmental samples. This method is unable to discriminate between infective and noninfective enterovirus particles; few clinical studies have compared real-time RT-PCR and viral culture. We wondered if the enterovirus genome quantification could be correlated to the infectivity. Methods and Results: We used the statistical approach to verify our hypotheses to correlate data, obtained by the standard method (most probable number of cytopathic units-MPNCU) and molecular test (real-time RT-PCR), on wastewater treatment plant samples. Chi-squared test was used, considering several cut-off values ('50'-'100'-'200' genome copy numbers), to determine statistical significance in comparison of the two methods. Chi-square value was not significant when cut-off of 50 (P = 0 center dot 103) and 100 (P = 0 center dot 178) was assumed but was significant with cut-off of 200 (P = 0 center dot 044). Conclusion: This limit, 200 genome copy, could be used as cut-off value to indicate enterovirus survival in environmental monitoring. Significant and Impact of the Study: To introduce a fast procedure that is able to compensate for disadvantages of cell culture method for viral environmental analyses.
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