期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 49, 期 2, 页码 260-266出版社
WILEY
DOI: 10.1111/j.1472-765X.2009.02653.x
关键词
Aspergillus versicolor; Cladosporium sphaerospermum; indoor mould; real-time PCR
资金
- French General Office of Health
- Direction Regionale des Affaires Sanitaires et Sociales (DRASS) of Franche-Comte
Aims: The aim of our study was to compare, using real-time (Rt) PCR, quantitative levels of five fungal species in three kinds of dwellings. Methods and Results: Three groups of homes were recruited: moisture-damaged homes (MDH, n = 30), allergic patient homes (APH, n = 25) and paired control homes (CH, n = 55). Five moulds with allergenic compounds or mycotoxin production characteristics (Cladosporium sphaerospermum, Penicillium chrysogenum, Aspergillus versicolor, Alternaria alternata and Stachybotrys chartarum) were quantified using Rt-PCR. Cycle threshold results were expressed in spore equivalent per volume or surface unit using a direct calculation based on a spore standard curve. MDH presented significantly higher amounts of DNA from C. sphaerospermum in both air and surface samples than CH (P < 0 center dot 001). APH presented slightly elevated amounts of DNA from A. versicolor in both air and surface samples, compared to CH (P < 0 center dot 05). Conclusion: Rt-PCR quantification of targeted fungal species is a rapid, reliable tool that could be included in a global indoor mould evaluation. Significance and Impact of the Study: Quantification of C. sphaerospermum using Rt-PCR can help to better target social service intervention in MDH. Quantification of A. versicolor DNA could be informative for characterization of APH.
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