期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 47, 期 6, 页码 600-607出版社
WILEY
DOI: 10.1111/j.1472-765X.2008.02473.x
关键词
Bacillus licheniformis; bacteriocin; fractional factorial design; media optimization
资金
- Council of Scientific Industrial Research (CSIR) New Delhi, India for financial assistance through the New Millennium Indian Technology Leadership Initiative [5/258/13/2002-NMITLI, 5/258/13A/2005-TNBD]
Enhancing production and characterization of a low-molecular-weight bacteriocin from Bacillus licheniformis MKU3. The culture supernatant of B. licheniformis MKU3 exhibited bacteriocin-like activity against Gram-positive and -negative bacteria and different fungi and yeast. SDS-PAGE analysis of the extracellular proteins of B. licheniformis MKU3 revealed a bacteriocin-like protein with a molecular mass of 1.5 kDa. This bacteriocin activity was found to be stable under a pH range of 3.0-10.0 and at temperatures up to 100 degrees C for 60 min, but inactivated by proteinase K, trypsin or pronase E. An experimental fractional factorial design for optimization of production medium resulted in a maximum activity of bacteriocin (11 000 AU ml(-1)) by B. licheniformis MKU3. A low-molecular-weight bacteriocin-like protein from B. licheniformis MKU3 exhibited a wide spectrum of antimicrobial activity against several Gram-positive bacteria, several fungi and yeast. A 3.6-fold increase in the production of bacteriocin was achieved using the culture medium optimized through a fractional factorial design. A bacteriocin with wide spectrum of activity against Gram-positive bacterial pathogens, filamentous fungi and yeast suggested its potential clinical use. Statistical method facilitated optimization of cultural medium for the improved production of bacteriocin.
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