期刊
LASERS IN MEDICAL SCIENCE
卷 24, 期 3, 页码 359-363出版社
SPRINGER LONDON LTD
DOI: 10.1007/s10103-008-0570-2
关键词
Multiphoton microscopy; Second harmonic generation; Two-photon excited fluorescence; Cervical tissue
资金
- National Natural Science Foundation of China [60508017, 60678054, 60711120031]
- Natural Science Foundation of the Fujian Province of China [2007J0007, C0720001]
- Program for New Century Excellent Talents in University [NCET-07-0191]
We demonstrate the application of multiphoton microscopy (MPM) based on two-photon excited fluorescence and second harmonic generation signals in imaging two-layered structures of cervical tissue in mice. It was found that MPM is effective for visualizing two-layered cervical microstructures, monitoring the metabolic activity and the density (nuclear-to-cytoplasmic) of epithelial cells, measuring the size of epithelial cell nuclei and the thickness of the epithelial layer, and quantifying collagen in the stroma. These results suggest that, with the advent of clinical portability of typical multiphoton endoscopes, the MPM technique has the potential to be applied in vivo to the clinical diagnosis and monitoring of cervical cancer.
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