The potential of Raman spectroscopy for the identification of biofilm formation by Staphylococcus epidermidis

We report on an investigation into a common problem in microbiology laboratories, which is associated with the difficulty of distinguishing/recognising different strains of the genus Staphylococcus. We demonstrate the potential, of, Raman spectroscopy as a rapid techniques allowing for the identification of different isolates for the detection of biofilin-positive and biofilm-negative Staphylococcus epidermidis strains, For this, the recorded Spectra were interpreted using the approach of principal component analysis (PCA). [GRAPHICS] Typical Raman spectra from individual bacterial colonies for three S. epidermidis strains (C) 2010 by Astro Ltd Published exclusively by WILEY-VCH Verlag GmbH & Co KGaA