期刊
LASER & PHOTONICS REVIEWS
卷 7, 期 5, 页码 758-778出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/lpor.201200060
关键词
Photoacoustic microscopy; multi-scale imaging; real-time imaging; functional imaging; molecular imaging; blood flow imaging; metabolic imaging; tumor imaging; gene expression imaging; brain imaging; nanoparticle imaging
资金
- National Institutes of Health (NIH) [R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, R01 CA159959]
Photoacoustic microscopy (PAM) is a hybrid in vivo imaging technique that acoustically detects optical contrast via the photoacoustic effect. Unlike pure optical microscopic techniques, PAM takes advantage of the weak acoustic scattering in tissue and thus breaks through the optical diffusion limit (similar to 1 mm in soft tissue). With its excellent scalability, PAM can provide high-resolution images at desired maximum imaging depths up to a few millimeters. Compared with backscattering-based confocal microscopy and optical coherence tomography, PAM provides absorption contrast instead of scattering contrast. Furthermore, PAM can image more molecules, endogenous or exogenous, at their absorbing wavelengths than fluorescence-based methods, such as wide-field, confocal, and multi-photon microscopy. Most importantly, PAM can simultaneously image anatomical, functional, molecular, flow dynamic and metabolic contrasts in vivo. Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.
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