4.6 Article

Mapping Single-Cell-Substrate Interactions by Surface Plasmon Resonance Microscopy

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LANGMUIR
卷 28, 期 37, 页码 13373-13379

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AMER CHEMICAL SOC
DOI: 10.1021/la301712h

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  1. NIH [R21RR026235]

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We report the imaging of the cell-substrate adhesion of a single cell with subcellular spatial resolution. Osmotic pressure was introduced to provide a controllable mechanical stimulation to the cell attached to a substrate, and high-resolution surface plasmon resonance microscopy was used to map the response of the cell, from which local cell-substrate adhesion was determined. In addition to high spatial resolution, the approach is noninvasive and fast and allows for the continuous mapping of cell substrate interactions and single-cell movements.

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