期刊
LANGMUIR
卷 26, 期 2, 页码 613-617出版社
AMER CHEMICAL SOC
DOI: 10.1021/la902564n
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资金
- Oak Ridge Institute for Science and Education
- U.S. Department of Energy
- U.S. Army Construction Engineering Research Laboratory
When pyrimidine-functionalized carbon nanotubes were incubated with single-stranded DNA ligase, formations of macroscopic aggregates were observed. Wet-cell transmission electron microscopy imaging revealed that the nanotubes were radially bound to form a 3D latticelike structure. These structures were not observed in control reactions lacking ligase or adenosine triphosphate. Raman spectroscopy analysis revealed no spectra indicative of carbon nanotubes in ligase-unamended controls; however, spectra were observed in radial breathing mode and in the G and G' bands in reactions containing ligase. Furthermore, the addition of deoxyribonuclease to the ligated reactions dispersed the aggregates, and a reduction in Raman spectral intensity was observed.
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