Layer-by-layer assembly was used to build thin films, consisting of multiple layers alternating cellulose nanocrystals and xyloglucan, benefiting from the strong non-electrostatic cellulose-xyloglucan interaction. Data from atomic force microscopy and neutron reflectivity showed that these well-defined films exhibited a thickness increasing linearly with the number of layers, without increase in surface roughness. These green nanocomposite films, reminiscent of plant cell wall, are composed of a regular stack of single layers of cellulose nanocrystals separated by very thin xyloglucan spacers. Such architecture differs from the one formed by cellulose/polycations multilayers, where the cellulose phase itself consists of a double layer.
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