期刊
LANCET INFECTIOUS DISEASES
卷 14, 期 6, 页码 489-497出版社
ELSEVIER SCI LTD
DOI: 10.1016/S1473-3099(14)70067-2
关键词
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资金
- National Basic Research Program (973 Program) [2007CB513100]
- National S & T Program in China [2012ZX10004220]
Background Schistosomiasis remains a highly prevalent and serious parasitic disease. A major factor preventing its effective management is the scarcity of effective diagnostic tools. We did a genome-wide identification of diagnostic protein markers for schistosome infection and assessed their diagnostic validity in a field study. Methods We predicted putative secreted proteins of Schistosoma japonicum (SjSPs) and expressed them as glutathione S-transferase (GST)-fusion proteins. The fusion proteins were arrayed on glutathione (GSH)-immobilised microplates and screened with serum samples from patients with schistosomiasis diagnosed by the Kato-Katz method. We further assessed an identified protein marker for sensitivity and specificity, first in infected serum samples collected from Jiangxi and Hunan Provinces, China, and then through a field study, done in two villages located in a high schistosomiasis-endemic area of the southeast of China. Findings Of 204 recombinant proteins, 35 yielded seropositive reactions, eight showed strong immunoreactivity, and only one (SjSP-13) reacted to the entire panel of 14 archived samples. The reactivity of SjSP-13 to 476 serum samples showed 90.4% (95% CI 86.5-93.5) sensitivity and 98.9% (95% CI 95.9-99.9) specificity. Of 1371 residents enrolled in a field study from Dec 6,2010, to June 23,2011, only 74 individuals were identified as being egg-positive, whereas 465 were diagnosed as positive by the SjSP-13-based ELISA kit (rSP13-ELISA). Of the 394 individuals found egg-negative but rSP13-ELISA-positive, 363 (92.4%) were confirmed to be positive for schistosome infection by PCR detection of S japonicum SjR2 retrotransposon. Interpretation The application of this sensitive, specific, and affordable rSP13-ELISA method should help reduce schistosomiasis transmission through targeted treatment of individuals, particularly with low intensity infections, and therefore support schistosomiasis control and elimination strategies.
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