期刊
LABORATORY INVESTIGATION
卷 92, 期 4, 页码 636-645出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/labinvest.2012.7
关键词
bowel; inflammation; macrophage; MRI; perfluorocarbon
资金
- National Institutes of Health [R01-CA134633]
- Pittsburgh NMR Center for Biomedical Research
- [P41-EB001977]
Macrophages have an important role in the pathogenesis of most chronic inflammatory diseases. A means of non-invasively quantifying macrophage migration would contribute significantly towards our understanding of chronic inflammatory processes and aid the evaluation of novel therapeutic strategies. We describe the use of a perfluorocarbon tracer reagent and in vivo F-19 magnetic resonance imaging (MRI) to quantify macrophage burden longitudinally. We apply these methods to evaluate the severity and three-dimensional distribution of macrophages in a murine model of inflammatory bowel disease (IBD). MRI results were validated by histological analysis, immunofluorescence and quantitative real-time polymerase chain reaction. Selective depletion of macrophages in vivo was also performed, further validating that macrophage accumulation of perfluorocarbon tracers was the basis of F-19 MRI signals observed in the bowel. We tested the effects of two common clinical drugs, dexamethasone and cyclosporine A, on IBD progression. Whereas cyclosporine A provided mild therapeutic effect, unexpectedly dexamethasone enhanced colon inflammation, especially in the descending colon. Overall, F-19 MRI can be used to evaluate early-stage inflammation in IBD and is suitable for evaluating putative therapeutics. Due to its high macrophage specificity and quantitative ability, we envisage F-19 MRI having an important role in evaluating a wide range of chronic inflammatory conditions mediated by macrophages. Laboratory Investigation (2012) 92, 636-645; doi:10.1038/labinvest.2012.7; published online 13 February 2012
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