期刊
LABORATORY ANIMALS
卷 48, 期 4, 页码 345-348出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/0023677214540881
关键词
centesis technique; cerebrospinal fluid; cisterna magna; general anaesthesia; piglets
资金
- Ricerca Fondamentale Orientata (RFO) grant from the University of Bologna
- Fondazione Telethon Funding Source: Custom
The collection of cerebrospinal fluid is necessary in order to determine its composition. It can then be used to diagnose various diseases. The aim of the study was to develop and optimize a technique for performing safe centesis for the collection of cerebrospinal fluid in piglets and its injection through the cisterna magna. The study was divided into three phases: (1) anatomical study of cadavers, (2) invivo application of the technique and (3) observation of recovery time. The proposed technique resulted in a safe puncture of the cisterna magna. The authors identified and confirmed the correspondence of the crista occipitalis and the wings of the atlas with the external landmarks on the cadaver by means of direct radiological visualization. The punctures were performed successfully at the first attempt in 11 out of 12 anaesthetized piglets. The technique herein described provides a reproducible safe and easy route for approaching the cisterna magna for cerebrospinal fluid collection, drug administration and gene delivery.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据