期刊
LAB ON A CHIP
卷 14, 期 15, 页码 2635-2641出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/C4LC00050A
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资金
- European Research Council ERC (eLab4life Project)
- Dutch Technolog Foundation STW (VENT grant)
- NWO - Netherlands Organization for Scientific Research (NanoNextNL) Spinoza Grant
In this article, we describe the development of a high throughput platform to spatially manipulate viable sperm for motility measurements and recovery of the best single sperm for fertilization purposes. Micro-contact printing was used to pattern islands of adhesive proteins (fibronectin) separated by sperm repellent species (Pluronic acid F-127) on commercially available polystyrene substrates. Following washing, arrays of viable single sperm were captured onto the islands demonstrating for the first time that sperm can be trapped by micro-contact printing with patterning efficiency of 90% while retaining 100% viability. These were then subjected to motility analysis whilst remaining spatially confined to the islands. Single sperm motility was assessed (n = 37) by software analysis measuring the number of rotations per second (degrees s(-1)). The assignment of array coordinates allows the more active single sperm to be easily identified and recovered by a simple micromaniputator pipette aspiration step with automated possibility for assisted reproductive technologies or further quality correlation analysis. Taken together, we show for the first time a technique to simultaneously screen thousands of viable single sperm for motility assessment while retaining the ability for single species recovery for enhanced fertilization purposes.
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