期刊
LAB ON A CHIP
卷 13, 期 14, 页码 2686-2692出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3lc50135c
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资金
- National Science Council of Taiwan [NSC 101-2628-E-007-011-MY3, NSC 100-2628-E-007-014-MY3]
- startup funds
- National Tsing Hua University, Taiwan
- Infectious Diseases Research and Education Center at National Taiwan University, Taiwan
This paper describes the development of nucleic acid detection in paper using a combination of commercial fluorescent probes and DNA ladders, and provides us with a better understanding of the interactions between double-stranded DNA (the amplified products in this study), fiber structures in paper, and fluorescent probes. The amplified products (the reverse-transcription and amplification of dengue virus serotype-2 RNA via RT-LAMP) in this study were subsequently fluorescently labeled in paper-based test zones (on our paper-based diagnostic device), thus fluorescent probes were used to perform the diagnosis of dengue fever, specific to serotype-2.
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