期刊
LAB ON A CHIP
卷 12, 期 3, 页码 595-601出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1lc20817a
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资金
- Canada Foundation for Innovation
- Ontario Research Fund
- Natural Sciences and Engineering Research Council of Canada
- Ontario Ministry for Research and Innovation
- NSERC CREATE
Gas bubbles present a frequent challenge to the on-chip investigation and culture of biological cells and small organs. The presence of a single bubble can adversely impair biological function and often viability as it increases the wall shear stress in a liquid-perfused microchannel by at least one order of magnitude. We present a microfluidic strategy for in-plane trapping and removal of gas bubbles with volumes of 0.1-500 nL. The presented bubble trap is compatible with single-layer soft lithography and requires a footprint of less than ten square millimetres. Nitrogen bubbles were consistently removed at a rate of 0.14 mu L min(-1). Experiments were complemented with analytical and numerical models to comprehensively characterize bubble removal for liquids with different wetting behaviour. Consistent long-term operation of the bubble trap was demonstrated by removing approximately 4000 bubbles during one day. In a case study, we successfully applied the bubble trap to the on-chip investigation of intact small blood vessels. Scalability of the design was demonstrated by realizing eight parallel traps at a total removal rate of 0.9 mu L min(-1) (measured for nitrogen).
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