期刊
LAB ON A CHIP
卷 12, 期 2, 页码 376-380出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1lc20698b
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资金
- Center for Cancer Research, National Cancer Institute, National Institutes of Health
- National Institutes of Health [GM069420]
- March of Dimes
- U.S. Department of Agriculture, Innovation and Economic Development Research Program
- Vilas Associate and Shaw scholar awards
- National Institutes of Health/National Library of Medicine [T15LM007359]
We synthesized customized double-stranded DNA microarrays including methyl-5-cytosine at CpG dinucleotides and produced all 163,555 possible 8-mers (un-, hemi-, and di-methylated) to gain insight into how methylation affects transcription factor binding. An antibody to methyl-5-cytidine showed greater binding to the methylated DNA, demonstrating efficient incorporation of methyl-5-cytosine into the synthesized DNA. In contrast, binding of the transcription factor CREB was inhibited by CpG methylation. This platform represents a powerful new technology to evaluate the effect of DNA methylation on protein binding in any sequence context.
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