期刊
LAB ON A CHIP
卷 12, 期 6, 页码 1151-1156出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2lc21096g
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- National Centre for Research and Development (NCBiR) [NR12-0027-04/2008]
The copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction was applied as the novel method of DNA immobilization on a modified solid support. The CuAAC click reaction enables the covalent binding of DNA modified with pentynyl groups at its 5'-end to azide-loaded slides. Click microarrays were produced using this approach and successfully employed in biological/model experiments.
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