Isolation of DNA aptamers using micro free flow electrophoresis

A micro free flow electrophoresis (mu FFE) device was used to select DNA aptamers for human immunoglobulin E (IgE). The continuous nature of mu FFE allowed 1.8 x 10(14) sequences to be introduced over a period of 30 min, a 300-fold improvement in library size over capillary electrophoresis based selections (CE-SELEX). Four rounds of selection were performed within four days. Aptamers with low nM dissociation constants for IgE were identified after a single round of mu FFE selection.